13 Dec 2016
F2, Firth Court
Professor Paul Guichard, University of Geneva, Switzerland
Dissecting the molecular composition and assembly of the centriole
The centriole is an evolutionary conserved organelle that composes the centrosome in cycling cells or gives rise to cilia in resting cells. Despite its crucial role in fundamental cell biology processes little is known about the 3D structure of this macromolecular complex. Conventional electron microscopy revealed the beauty as well as the complexity of the centriole ultrastructure and pointed out its overall evolutionarily structural conservation between species. However, exquisite details of the centriolar architecture at high resolution are lacking to understand the basis of centriole assembly. Using in vitro assay coupled to cryo-microscopy and tomography, quantitative mass spectrometry and super-resolution microscopy I will present how we are dissecting the molecular architecture and the assembly of the centriole.