05 April 2016
Lecture Theatre F2, Firth Court
Dr Paul Verkade, Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology, Pharmacology & Neuroscience, University of Bristol, UK
Correlative Light Electron Microscopy: 1 + 1 = 3
Abstract: Correlative Light Electron Microscopy (CLEM) combines the strengths of light and electron microscopy in one experiment and the sum of such an experiment should provide more data / insight than each technique alone (1 + 1 = 3). There are many ways to perform a CLEM experiment but the experimental set up should primarily depend on the biological question to be answered. Sometimes a fairly simple approach suffices to answer the question. In other cases however it requires the development of new tools and instruments to adequately do so. In my presentation I will highlight this approach through a number of examples.
- Olmos, Y. L. Hodgson, J. Mantell, P. Verkade, and J.G. Carlton (2015). ESCRT-III controls nuclear envelope reformation. Nature, 522: 236–239.
- Hodgson L, D. Nam, J. Mantell, A. Achim, and P. Verkade. (2014). Retracing in Correlative Light Electron Microscopy: Where is My Object of Interest? Methods in Cell Biology, Volume 124: Correlative Light and Electron Microscopy, 1-21.
- Hodgson L, J. Tavaré, and P. Verkade. (2014). Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking. Protoplasma. 251:403-416.
- Brown, E., J., Van Weering, T. Sharp, J. Mantell, and P. Verkade (2012). Capturing endocytic segregation events with HPF-CLEM. Methods in Cell Biology, Volume 111: Correlative Light and Electron Microscopy, 175-201.